CLINICAL BIOCHEMISTRY AND CLINICAL MOLECULAR BIOLOGY

Università degli Studi
"G. d'Annunzio"

Chieti - Pescara

General Module

Course Sheet

Course code:

TF11A2B

Academic Year of enrolment:

2019

Year of supply:

2019/2020

Type of Course:

Basic

Degree:

Course of 1st Cycle Degree in CARDIOCIRCULATORY AND CARDIOVASCULAR PERFUSION TECHNIQUES

Disciplinary Sector:

Clinical Biochemistry and Biology

Is part of:

BASIC SCIENCES

Location:

CHIETI

Credits:

Programme Year:

Professor and Collaborators:

PIERAGOSTINO DAMIANA

Cycle:

First semester

Obligatory attendance:

Yes

Hours of classroom activity:

Individual study hours:

Prerequisites:

Basic knowledges of Organic Chemistry and Biochemistry Propaedeutics

Objectives

Knowledge of the metabolic specializations of the main organs and systems and related biochemical tests used for diagnosis.

Contents

Fundamentals of laboratory medicine;
Clinical biochemistry Analysis with particular reference to the alterations of the biochemical picture in organs and systems diseases .
Analytical techniques in clinical biochemistry and laboratory medicine

Extended Syllabus

General concepts of clinical biochemistry and laboratory medicine: reference range, diagnostic threshold, sensitivity and specificity, ROC curves, Positive and Negative predictive values. Biological, pre-analytical, analytical and post-analytical variables. Biomarker concept: specific and general biomarkers.
Statistical tools . Analytical performance evaluation (Accuracy, Precision, LOD, LOQ), Diagnostic tests and ROC Curves analysis. “Brain-to-Brain loop” process. Pre-analytical step, Biological Variability, Sample Variability, Indicators of Quality.
The Blood: Arterial, Venous and Capillary. Arterial blood gas (ABG) test: pathophysiology, oxygenation status and acid–base balance. Diagnostic Haematology: Red blood cells (RBCs), white blood cells (WBCs), and platelets. Blood Smear, Hematocrit (Hct), Hemoglobin (Hgb), Mean Corpuscular Volume (MCV), Mean Corpuscolar Haemoglobin (MCH ), Mean Corpuscular Hemoglobin Concentration (MCHC), Red Cell Distribution Width (RDW). Platelet indices (PI): mean platelet volume (MPV), platelet distribution width (PDW), plateletcrit (PCT).
Hemoglobin: structure, oxygen bonding, binding cooperativity, carbon dioxide transport and Bohr effect, 2,3-bisphosphoglycerate, allosteric regulation. Pathological hemoglobins.
Dyslipidaemias. Plasma lipoproteins (LDL, VLDL, Chylomicrons, HDL, Lp (a)), LDL Receptor, LPL. Lipoprotein metabolism, exogenous and endogenous. Dyslipidemias, Fredrickson classification, Pathogenetic classification. Lipid profile and diagnosis.
Diagnostic Enzyme. Plasma concentration of enzymes (Release and Clearance). Muscle, Liver, Pancreatic, Bone and Cardiac Enzymes. Techniques for measuring the enzymatic activity of the main enzymes of diagnostic interest.
Serum protein electrophoresis. Electrophoretic techniques and application to plasma cell dyscrasias.
Kidney: urine formation and laboratory diagnostics, biomarkers of kidney diseases, acid-base balance, classification of kidney diseases. Chemical-physical and morphological examination of urine.
Diabetes. Classification, Blood sugar, diagnosis and complications.

Recommended Bibliography

Books
1. M. Ciaccio, G. Lippi- Biochimica Clinica e Medicina di Laboratorio. Edises
2. Medicina di laboratorio Giorgio Federici McGraw-Hill

Teaching Methods

Traditional Lesson
weekly student reception

Evaluation methods

Verification of learning:

Oral exam
At the end of the oral examination a judgment will be expressed as reflected by the vote in thirtieths:
A) excellent knowledge and understanding of the topics, reasoning skills and logical integration of the acquired knowledge and insertion in the context of Clinical Biochemistry. Rating: 30/30.
If the student, who has achieved this evaluation, has shown wide autonomy of judgment, high communication skills and autonomous learning skills, the laude will be proposed.
B) Good knowledge and understanding of the topics with some minor inaccuracies and with a good capacity for logical integration and judgment, and application Rating: 27-29 / 30
C) Discrete knowledge and understanding of the topics and, despite some gaps in the ability to integrate and apply knowledge. Rating: 24-26 / 30
D) Knowledge and understanding of the arguments fully sufficient, although there are clear gaps, which are reflected in a reduced capacity for integration, logic and application. Rating 21-23 / 30
E) Knowledge and understanding of the topics with serious and evident gaps, with limited integrative and applicative skills, but which also allow the passing of the exam. Rating 18-20 / 30

Università degli Studi
"G. d'Annunzio"

Chieti - Pescara

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