ANALYSES OF MEDICINES

Università degli Studi
"G. d'Annunzio"

Chieti - Pescara

Single discipline educational activity

Course Sheet

Course code:

00010

Academic Year of enrolment:

2020

Year of supply:

2021/2022

Type of Course:

Characterising

Degree:

Course of Single Cycle Degree 5 years in MEDICINAL CHEMISTRY AND PHARMACEUTICAL TECHNOLOGY

Disciplinary Sector:

Pharmaceutical Chemistry

Language:

Italian

Location:

CHIETI

Credits:

Programme Year:

Professor and Collaborators:

AGAMENNONE Mariangela

Cycle:

Second semester

Obligatory attendance:

Yes

Minimum hours of attendance:

Hours of classroom activity:

Individual study hours:

Prerequisites:

For a comprehensive understanding of the topics of the course, a strong basis of General and Inorganic Chemistry is needed. Therefore, students are warmly encouraged to take that exam before starting the course.
The exam can be taken after passing the Analytical Chemistry exam.

Objectives

At the end of the course the student shall reach the following objectives:
- Comprehension of chemical equilibria mechanism and management of multiple equilibria in water solution.
- Knowledge of chemical behavior of main cations and anions.
- Ability to setup a separation and identification protocol of simple mix of inorganic compounds.
- Knowledge of the pharmaceutical, pharmacological and toxicological role of most important cations and anions.
- Learning of principal lab operations, through practical lab experiences.
- Knowledge of most important safety rules in the lab practice.

Contents

- Introduction to the qualitative inorganic analysis
- General aspects of the laboratory procedures
- Safety rules in the chemistry lab
- Chemical equilibria in water solution
- The Italian and European Pharmacopoeia.
- Systematic analysis of cations
- Analysis of cations and anions
- Cations and anions of pharmaceutical and toxicological interest
- Purification methods
- Spectroscopic methods of analysis
- Lab experiences

Extended Syllabus

Introduction to the qualitative inorganic analysis
Properties and characteristics of inorganic compounds.

General aspects of the laboratory procedures
Main semimicro analysis techniques: evaporation and boiling, calcination, melting, filtration, centrifugation, washing of precipitates.

Safety rules in the chemistry lab

Chemical equilibria in water solution
Acid-base, solubility, redox and coordination equilibria.

The Italian and European Pharmacopeia.

Analysis of cations and anions
Analysis of main cations and anions of pharmaceutical interest.
Preliminary assays. Flame test.
Application of solution multiple equilibria to separate and identify cations and anions. Separation examples.
Identification assays and limit test of inorganic species, described in the Pharmacopeia.

Cations and anions of pharmaceutical and toxicological interest
Biochemical, pharmaceutical and toxicological aspects of the inorganic substances.

Purification methods
Chromatography: general aspects. Ion exchange chromatography.

Spectroscopic methods of analysis
General aspects; Atomic absorption and emission spectroscopy. ICP-AES and ICP-MS.

Lab experiences
Fundamental procedures: precipitation, filtration, centrifugation.
Flame test.
Separation and identification of cations and anions as by systematic analysis and as by Pharmacopeia. Separation of ionic species through ion exchange chromatography.

Recommended Bibliography

- Chimica analitica qualitativa – A. Araneo. Casa Editrice Ambrosiana, Milano
- L’analisi qualitativa in chimica farmaceutica e tossicologica inorganica – P. Barbetti, M. G. Quaglia. Galeno Editrice
- Analisi chimica strumentale R. Cozzi, P. Protti, T. Ruaro. Zanichelli Editore, Bologna
- Italian Official Pharmacopoeia XII Ed. and Suppl.
- European Pharmacopoeia X Ed. and Suppl.

Methods of Provision

Conventional

Teaching Methods

The course is organized in 32 hours of taught-class and 24 hours of lab practice. During the lectures, the theoretical aspects of the analysis of pharmaceutically relevant inorganic compounds will be discussed.
The lab practice, single seat, are organized so that the student can deal with lab practice and carry out the analysis explained in the taught-class

Evaluation methods

Verification of learning:

The final exam consists of an oral examination lasting 30-40 minutes, aimed to verify the comprehension of general concepts of the analysis in water solution, also through the resolution of exercises. Gaps in the basic knowledge of General Chemistry concepts will not be tolerated. The exam will be completed by verifying the knowledge of the pharmaceutical and toxicological role of inorganic compounds and instrumental techniques of analysis. The result of the lab tests will contribute to the final evaluation.

Università degli Studi
"G. d'Annunzio"

Chieti - Pescara

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