THEORIC LESSONS
Organic Analysis. Qualitative analysis of organic compounds. Preliminary evaluation: organoleptic analysis, structure chemical-physical properties relationships. Drugs solubility. Structure-Solubility relationships. Melting point. Eutectics. Polymorphism and techniques for the characterization of the polymorphs. Boiling point. Refractometry. Polarimetry. Optical Rotatory Dispersion. Circular Dichroism. Qualitative elemental analysis. Functional organic analysis and FU drugs identification procedures.
Separation and Purification Methods. Crystallization. Filtration. Liquids purification: Ideal and real mixture. Azeotropics. Distillation. Sublimation. Liquid- Liquid Extraction. Continuos extraction. Soxhlet apparatus. Theoretical basis of chromatography. Planar and on column chromatography. Monodimensional and bidimensional HPLC. Gas-chromatography. Supercritical Fluids chromatography. Chromatographic resolution of racemic mixture. Method validation. Chromatographic quantitative analysis.
Capillary Electrophoresis. Theoretical basis. Instrument. Analytical parameters: efficiency, mobility, resolution. EOF. Joule effect. CZE. Chiral selectors.
Determination of drugs physico-chemical parameters. Evaluation of acid dissociation constant (pKa), lipophilicity (logP) solubility, chemical stability.
CLASSROOM EXERCITATIONS
Analysis of HPLC-Uv chromatograms: evaluation of the column and the mobile phase composition on efficiency, selectivity, retention and resolution. Separations in gradient mode. Optimization of an impurity profiling method.
LABORATORY EXERCITATIONS
Solubility evaluation of organic and organometallic compounds. Identification of the unsaturated compound and of the main functional groups in selected compounds. M.p. evaluation. FU compounds identification. Liquid-Liquid Extraction of a given mixture. TLC. Crystallization. Column chromatography. Extraction of API from drugs.
